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验证数据展示

  • WB analysis of BxPC-3 using 83733-3-RR (same clone as 83733-3-PBS)

    WB analysis of BxPC-3 using 83733-3-RR (same clone as 83733-3-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • WB analysis using 83733-3-RR (same clone as 83733-3-PBS)

    WB analysis using 83733-3-RR (same clone as 83733-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 83733-3-RR (same clone as 83733-3-PBS)

    IHC staining of human colon cancer using 83733-3-RR (same clone as 83733-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included
  • IHC staining of human stomach cancer using 83733-3-RR (same clone as 83733-3-PBS)

    IHC staining of human stomach cancer using 83733-3-RR (same clone as 83733-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83733-3-RR (ARID1A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARID1A antibody included
  • IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00714-2

    Cytometric bead array standard curve of MP00714-2

    Cytometric bead array standard curve of MP00714-2, ARID1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83733-3-PBS. Detection antibody: 83733-2-PBS. Standard: Ag30749. Range: 1.25 -80 ng/mL.

  • Affinity and Kinetic Characterization of 83733-3-RR

    Affinity and Kinetic Characterization of 83733-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

ARID1A Recombinant antibody, PBS Only (Capture)

ARID1A Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83733-3-PBS

产品说明书

CloneNo. 240690F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARID1A Ready-To-Use IHC Kit. ARID1A primary antibody included.

产品信息

83733-3-PBS targets ARID1A as part of a matched antibody pair:

MP00714-2: 83733-3-PBS capture and 83733-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 ARID1A fusion protein Ag30749 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 AT rich interactive domain 1A (SWI-like)
别名 BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A
计算分子量 242 kDa
观测分子量250-260 kDa
GenBank蛋白编号NM_006015
基因名称 ARID1A
Gene ID (NCBI) 8289
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14497
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.