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验证数据展示

  • WB analysis using 23406-1-AP

    WB analysis using 23406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23406-1-AP (ARID2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 23406-1-AP

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 23406-1-AP

    IHC staining of mouse testis using 23406-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARID2 Polyclonal antibody

ARID2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23406-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23406-1-AP targets ARID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 ARID2 fusion protein Ag19968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 AT rich interactive domain 2 (ARID, RFX-like)
别名 Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2
计算分子量 1835 aa, 197 kDa
观测分子量200-230 kDa
GenBank蛋白编号BC090062
基因名称 ARID2
Gene ID (NCBI) 196528
RRIDAB_2918080
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68CP9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARID2 antibody 23406-1-APDownload protocol
IHC protocol for ARID2 antibody 23406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Am Heart Assoc

Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2

Authors - Runtai Chen
View Article