验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, A549 cells, K-562 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue, human cervical cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy ARID4B Ready-To-Use IHC Kit. ARID4B primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24499-1-AP targets ARID4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ARID4B fusion protein Ag21462 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | AT rich interactive domain 4B (RBP1-like) |
| 别名 | RBBP1L1, Breast cancer-associated antigen BRCAA1, BRCAA1, AT-rich interactive domain-containing protein 4B, ARID domain-containing protein 4B |
| 计算分子量 | 1312 aa, 148 kDa |
| 观测分子量 | 100 kDa, 200 kDa |
| GenBank蛋白编号 | BC130418 |
| 基因名称 | ARID4B |
| Gene ID (NCBI) | 51742 |
| RRID | AB_2879575 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q4LE39 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARID4B antibody 24499-1-AP | Download protocol |
| IHC protocol for ARID4B antibody 24499-1-AP | Download protocol |
| IF protocol for ARID4B antibody 24499-1-AP | Download protocol |
| IP protocol for ARID4B antibody 24499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer. | ||
Theranostics SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma. | ||
Cell Death Dis DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways. | ||
Br J Nutr Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway.
| ||
Cancer Lett Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance |
