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验证数据展示

  • WB analysis of T-47D using 67384-1-Ig

    WB analysis of T-47D using 67384-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67384-1-Ig (ARID4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67384-1-Ig

    IHC staining of human breast cancer using 67384-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67384-1-Ig (ARID4B antibody) at dilution of 1:1000 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ARID4B antibody included

ARID4B Monoclonal antibody

ARID4B Monoclonal Antibody for WB, ELISA
Cat No. 67384-1-Ig

Print datasheet

CloneNo. 1H3E2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Synonyms

ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, MDA-MB-231 cells
Planning an IHC experiment? We recommend our IHCeasy ARID4B Ready-To-Use IHC Kit. ARID4B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67384-1-Ig targets ARID4B in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ARID4B fusion protein Ag21485 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name AT rich interactive domain 4B (RBP1-like)
Synonyms ARID4B, BRCAA1, DKFZp313M2420, RBBP1L1, RBP1L1, SAP180
Calculated Molecular Weight 1312 aa, 148 kDa
GenBank Accession NumberBC130418
Gene Symbol ARID4B
Gene ID (NCBI) 51742
RRIDAB_2918489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ4LE39
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

实验方案

Product Specific Protocols
WB protocol for ARID4B antibody 67384-1-IgDownload protocol
IHC protocol for ARID4B antibody 67384-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols