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  • Featured Product
  • KD/KO Validated

ARIH1 Polyclonal antibody

ARIH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14949-1-AP

Print datasheet

Synonyms

H7 AP2, EC:2.3.2.31, E3 ubiquitin-protein ligase ARIH1, ARI-1, ARI 1

验证数据展示

  • WB analysis using 14949-1-AP

    WB analysis using 14949-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14949-1-AP (ARIH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 14949-1-AP

    IHC staining of mouse small intestine using 14949-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 14949-1-AP (ARIH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 14949-1-AP

    IHC staining of mouse small intestine using 14949-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 14949-1-AP (ARIH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14949-1-AP targets ARIH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ARIH1 fusion protein Ag6760 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila)
Synonyms H7 AP2, EC:2.3.2.31, E3 ubiquitin-protein ligase ARIH1, ARI-1, ARI 1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC051877
Gene Symbol ARIH1
Gene ID (NCBI) 25820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4X5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767).

实验方案

Product Specific Protocols
WB protocol for ARIH1 antibody 14949-1-APDownload protocol
IHC protocol for ARIH1 antibody 14949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity

Authors - Lei Chu
  • KD Validated
View Article
humanWB

bioRxiv

MDA5 ISGylation is crucial for immune signaling to control viral replication and pathogenesis

Authors - Lucky Sarkar
  • KD Validated
View Article