验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, Jurkat cells, LNCaP cells, NIH/3T3 cells |
Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 2 publications below |
产品信息
14949-1-AP targets ARIH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | ARIH1 fusion protein Ag6760 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila) |
别名 | H7 AP2, EC:2.3.2.31, E3 ubiquitin-protein ligase ARIH1, ARI-1, ARI 1 |
计算分子量 | 64 kDa |
观测分子量 | 64 kDa |
GenBank蛋白编号 | BC051877 |
基因名称 | ARIH1 |
Gene ID (NCBI) | 25820 |
RRID | AB_3085447 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y4X5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARIH1 (Ariadne-1 homolog) is a member of the Ariadne family of E3 ubiquitin ligases with a cognate E2 enzyme UBCH79,10,11. ARIH1 plays a role in protein translation regulation in response to DNA damage and to ubiquitinate EIF4E2 (PMID: 15236971, PMID: 1532592, PMID: 24076655, PMID: 27565346, PMID: 23707686). ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation (PMID: 33879767).
实验方案
Product Specific Protocols | |
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WB protocol for ARIH1 antibody 14949-1-AP | Download protocol |
IHC protocol for ARIH1 antibody 14949-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep HERC5-catalyzed ISGylation potentiates cGAS-mediated innate immunity
| ||
bioRxiv MDA5 ISGylation is crucial for immune signaling to control viral replication and pathogenesis
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