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验证数据展示

  • WB analysis using 22723-1-AP (same clone as 22723-1-PBS)

    WB analysis using 22723-1-AP (same clone as 22723-1-PBS)

    rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 22723-1-AP (same clone as 22723-1-PBS)

    WB analysis of mouse brain using 22723-1-AP (same clone as 22723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ARK5 Polyclonal antibody, PBS Only

ARK5 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22723-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

22723-1-PBS targets ARK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ARK5 fusion protein Ag18612 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 NUAK family, SNF1-like kinase, 1
别名
计算分子量 661 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC152462
基因名称 ARK5
Gene ID (NCBI) 9891
RRIDAB_2879157
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO60285
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.