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ARK5 Recombinant antibody

ARK5 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242411F1

Cat no : 84893-5-RR

Print datasheet

Synonyms

NUAK1, NUAK family SNF1-like kinase 1, NUAK 1, EC:2.7.11.1, AMPK-related protein kinase 5

验证数据展示

  • IF Staining of A431 using 84893-5-RR

    IF Staining of A431 using 84893-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NUAK1 antibody (84893-5-RR, Clone: 242411F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84893-5-RR targets ARK5 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ARK5 fusion protein Ag18832 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NUAK family, SNF1-like kinase, 1
Synonyms NUAK1, NUAK family SNF1-like kinase 1, NUAK 1, EC:2.7.11.1, AMPK-related protein kinase 5
Calculated Molecular Weight 661 aa, 74 kDa
GenBank Accession NumberBC152462
Gene Symbol ARK5
Gene ID (NCBI) 9891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60285
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for ARK5 antibody 84893-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols