验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, PC-3 cells, human brain tissue, mouse testis tissue, rat brain tissue, HeLa cells, rat testis tissue |
Positive IHC detected in | human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MDCK cells, IMCD3 cells, hTERT-RPE1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10961-1-AP targets ARL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat, canine |
文献引用反应性 | human, mouse |
免疫原 | ARL3 fusion protein Ag1406 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ADP-ribosylation factor-like 3 |
别名 | ADP-ribosylation factor-like protein 3, ADP ribosylation factor like 3 |
计算分子量 | 20 kDa |
观测分子量 | 20 kDa, 25 kDa |
GenBank蛋白编号 | BC009841 |
基因名称 | ARL3 |
Gene ID (NCBI) | 403 |
RRID | AB_2274220 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P36405 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.
实验方案
Product Specific Protocols | |
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WB protocol for ARL3 antibody 10961-1-AP | Download protocol |
IHC protocol for ARL3 antibody 10961-1-AP | Download protocol |
IF protocol for ARL3 antibody 10961-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Commun Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation. | ||
Nat Commun Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.
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Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. | ||
Dev Cell The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK. |