验证数据展示

WB analysis using 10961-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of IMCD3 cells using 10961-1-AP
IF result of 10961-1-AP (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus).
WB analysis of HeLa using 10961-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 10961-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with ARL3 antibody included
IHC staining of human testis using 10961-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with ARL3 antibody included
IF Staining of hTERT-RPE1 using 10961-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MDCK using 10961-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

ARL3 Polyclonal antibody

ARL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10961-1-AP

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Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Synonyms

ADP-ribosylation factor-like protein 3, ADP ribosylation factor like 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (17)
实验方案

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MSDS

经过测试的应用

Positive WB detected in	mouse brain tissue, PC-3 cells, human brain tissue, mouse testis tissue, rat brain tissue, HeLa cells, rat testis tissue
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MDCK cells, IMCD3 cells, hTERT-RPE1 cells

Planning an IHC experiment? We recommend our IHCeasy ARL3 Ready-To-Use IHC Kit. ARL3 primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 12 publications below
IHC	See 2 publications below
IF	See 6 publications below
IP	See 1 publications below

产品信息

10961-1-AP targets ARL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications	WB, IHC, IF/ICC, ELISA Application Description
Cited Applications	WB, IHC, IF, IP
Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse
Immunogen	ARL3 fusion protein Ag1406 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ADP-ribosylation factor-like 3
Synonyms	ADP-ribosylation factor-like protein 3, ADP ribosylation factor like 3
Calculated Molecular Weight	20 kDa
Observed Molecular Weight	20 kDa, 25 kDa
GenBank Accession Number	BC009841
Gene Symbol	ARL3
Gene ID (NCBI)	403
RRID	AB_2274220
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36405
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

实验方案

Product Specific Protocols
WB protocol for ARL3 antibody 10961-1-AP	Download protocol
IHC protocol for ARL3 antibody 10961-1-AP	Download protocol
IF protocol for ARL3 antibody 10961-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Nat Commun A stress-induced cilium-to-PML-NB route drives senescence initiation Authors - Xiaoyu Ma View Article
human	WB,IF	Nat Commun Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation. Authors - Antonios D Konitsiotis View Article
mouse	WB,IF, IP	Nat Commun Arl3 and LC8 regulate dissociation of dynactin from dynein. Authors - Mingyue Jin KD Validated View Article
mouse	WB	Nat Commun Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism. Authors - Hyunho Kim KD Validated View Article
human	WB	Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. Authors - Humbert Melissa C MC View Article
human	IF	Dev Cell The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK. Authors - Louise A Stephen View Article

验证数据展示

WB analysis using 10961-1-AP

IF Staining of IMCD3 cells using 10961-1-AP

WB analysis of HeLa using 10961-1-AP

IHC staining of human testis using 10961-1-AP

IHC staining of human testis using 10961-1-AP

IF Staining of hTERT-RPE1 using 10961-1-AP

IF Staining of MDCK using 10961-1-AP

ARL3 Polyclonal antibody

ARL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

A stress-induced cilium-to-PML-NB route drives senescence initiation

Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.

Arl3 and LC8 regulate dissociation of dynactin from dynein.

Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.