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验证数据展示

  • WB analysis of HeLa using 68421-1-Ig

    WB analysis of HeLa using 68421-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit testis using 68421-1-Ig

    WB analysis of rabbit testis using 68421-1-Ig

    rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat retina using 68421-1-Ig

    WB analysis of rat retina using 68421-1-Ig

    rat retina tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 68421-1-Ig

    IF Staining of hTERT-RPE1 using 68421-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL3 antibody (68421-1-Ig, Clone: 2E5B12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

ARL3 Monoclonal antibody

ARL3 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68421-1-Ig

产品说明书

CloneNo. 2E5B12

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat, rabbit

应用

WB, IF/ICC, ELISA

别名

ADP ribosylation factor like 3, ARFL3, ARL3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rabbit testis tissue, rat retina tissue
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy ARL3 Ready-To-Use IHC Kit. ARL3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68421-1-Ig targets ARL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, rat, rabbit
免疫原 ARL3 fusion protein Ag21387 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 3
别名 ADP ribosylation factor like 3, ARFL3, ARL3
计算分子量 20 kDa
观测分子量 20-24 kDa
GenBank蛋白编号BC009841
基因名称 ARL3
Gene ID (NCBI) 403
RRIDAB_3085136
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP36405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

实验方案

Product Specific Protocols
WB protocol for ARL3 antibody 68421-1-IgDownload protocol
IF protocol for ARL3 antibody 68421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols