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ARL4D Polyclonal antibody

ARL4D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 15267-1-AP

Print datasheet

Synonyms

ARF4L, ARL4D, ARL6

验证数据展示

  • WB analysis using 15267-1-AP

    WB analysis using 15267-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15267-1-AP (ARL4D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse retina tissue, Y79 cells, rat retina tiissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15267-1-AP targets ARL4D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ARL4D fusion protein Ag7369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosylation factor-like 4D
Synonyms ARF4L, ARL4D, ARL6
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC000043
Gene Symbol ARL4D
Gene ID (NCBI) 379
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49703
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL4D (ADP-ribosylation factor-like protein 4D) is also named as ARF4L. ARL4D is a developmentally regulated member of the ADP-ribosylation factor/ARF-like protein (ARF/ARL) family of Ras-related GTPases. ARL4D interacts with the C-terminal pleckstrin homology (PH) and polybasic c domains of cytohesin-2/ARNO in a GTP-dependent manner. Localization of ARL4D at the plasma membrane is GTP- and N-terminal myristoylation-dependent. ARL4D(Q80L), a putative active form of ARL4D, induced accumulation of cytohesin-2/ARNO at the plasma membrane  (PMID: 17804820).

实验方案

Product Specific Protocols
WB protocol for ARL4D antibody 15267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols