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ARL5B Polyclonal antibody

ARL5B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11694-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

ARL8, ADP-ribosylation factor-like protein 8, ADP-ribosylation factor-like protein 5B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inU2OS cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11694-1-AP targets ARL5B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human
文献引用反应性human, mouse
免疫原 ARL5B fusion protein Ag2309 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ADP-ribosylation factor-like 5B
别名 ARL8, ADP-ribosylation factor-like protein 8, ADP-ribosylation factor-like protein 5B
计算分子量 179 aa, 20 kDa
观测分子量 20 kDa
GenBank蛋白编号BC024163
基因名称 ARL5B
Gene ID (NCBI) 221079
RRIDAB_2058835
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96KC2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARL5B, also known as ADP ribosylation factor-like GTPase 5B, is a small G protein that is localized to the trans-Golgi network (TGN) in mammalian cells. It plays a significant role in the regulation of retrograde membrane transport from endosomes back to the TGN. This function is particularly important for the maintenance of cellular membrane traffic and the organization of the Golgi apparatus.

实验方案

Product Specific Protocols
WB protocol for ARL5B antibody 11694-1-APDownload protocol
IHC protocol for ARL5B antibody 11694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IP

J Biol Chem

Negative regulation of MDA5-dependent antiviral innate immune responses by Arl-like protein 5B.

Authors - Yuichi Kitai
  • KO Validated
mouseWB

J Cell Biochem

GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.

Authors - Chun-Xiang Zhou
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