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BMAL1 Polyclonal antibody

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA and More (4)

Conjugate

Unconjugated

Publications

Cat no : 14268-1-AP

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Synonyms

ARNTL, ARNT3, bHLHe5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1

验证数据展示

WB analysis using 14268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 14268-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
FC experiment of HeLa using 14268-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARNTL (14268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of HeLa using 14268-1-AP
IP result of anti-BMAL1 (IP:14268-1-AP, 4ug; Detection:14268-1-AP 1:300) with HeLa cells lysate 1600ug.
IF Staining of U-87 MG using 14268-1-AP
Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using BMAL1 antibody (14268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Cat No. 14268-1-AP

Size

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经过测试的应用

Positive WB detected in	HT-1080 cells, 3T3-L1 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	U-87 MG cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy BMAL1/ARNTL Ready-To-Use IHC Kit. BMAL1/ARNTL primary antibody included.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 9 publications below
WB	See 51 publications below
IHC	See 14 publications below
IF	See 8 publications below
IP	See 1 publications below
CoIP	See 2 publications below
ChIP	See 5 publications below

产品信息

14268-1-AP targets BMAL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP, chIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	BMAL1 fusion protein Ag5586 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	aryl hydrocarbon receptor nuclear translocator-like
Synonyms	ARNTL, ARNT3, bHLHe5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1
Calculated Molecular Weight	69 kDa
Observed Molecular Weight	69-75 kDa
GenBank Accession Number	BC041129
Gene Symbol	ARNTL
Gene ID (NCBI)	406
RRID	AB_2878037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O00327
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

实验方案

Product Specific Protocols
WB protocol for BMAL1 antibody 14268-1-AP	Download protocol
IF protocol for BMAL1 antibody 14268-1-AP	Download protocol
IP protocol for BMAL1 antibody 14268-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Mol Cell CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis. Authors - Ran Lin KD Validated View Article
human	WB	Nat Commun The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock. Authors - Yi Liu View Article
mouse	WB	J Pineal Res Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors Authors - Yao Zuo View Article
	WB	Sci Total Environ Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario Authors - Fangnon Firmin Fangninou View Article
mouse	IHC	Theranostics Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia. Authors - Shuai Wang View Article
mouse	WB	Environ Pollut Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction. Authors - Feifei Shi View Article

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BMAL1 Polyclonal antibody

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 14268-1-AP

WB analysis using 14268-1-AP

FC experiment of HeLa using 14268-1-AP

IP experiment of HeLa using 14268-1-AP

IF Staining of U-87 MG using 14268-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.

The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.

Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors

Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario

Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.

Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.

Flow Cytometry Perm Buffer (10X)