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ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Publications

Cat no : 15058-1-AP

Print datasheet

Synonyms

p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2

验证数据展示

WB analysis using 15058-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included
IHC staining of human breast cancer using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included
IF Staining of HeLa using 15058-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 15058-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
WB analysis of MCF-7 using 15058-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15058-1-AP
IP result of anti-ARPC2 (IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug.
IHC staining of human normal colon using 15058-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ARPC2 antibody included

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Cat No. 15058-1-AP

Size

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经过测试的应用

Positive WB detected in	HT-1080 cells, HeLa cells, mouse skeletal muscle tissue, PC-3 cells, 3T3-L1 cells, NIH/3T3 cells, MCF-7 cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 6 publications below
IF	See 1 publications below
IP	See 1 publications below

产品信息

15058-1-AP targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	ARPC2 fusion protein Ag7041 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	actin related protein 2/3 complex, subunit 2, 34kDa
Synonyms	p34-ARC, p34 Arc, ARC34, Actin-related protein 2/3 complex subunit 2
Calculated Molecular Weight	34 kDa
Observed Molecular Weight	34 kDa
GenBank Accession Number	BC000590
Gene Symbol	ARPC2
Gene ID (NCBI)	10109
RRID	AB_2059787
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15144
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for ARPC2 antibody 15058-1-AP	Download protocol
IHC protocol for ARPC2 antibody 15058-1-AP	Download protocol
IF protocol for ARPC2 antibody 15058-1-AP	Download protocol
IP protocol for ARPC2 antibody 15058-1-AP	Download protocol
FC protocol for ARPC2 antibody 15058-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Dev Cell Aberrant cortex contractions impact mammalian oocyte quality Authors - Elvira Nikalayevich View Article
	WB	Genes Dev Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein Authors - Megan L Norris View Article
human	WB	J Cell Biol α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3. Authors - Nikki R Paul KD Validated View Article
human	IP	EMBO Rep WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis. Authors - Kailiang Zhao View Article
human	WB	J Neurosci The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse. Authors - Avanti Gokhale View Article
human	WB, IF	Front Cell Dev Biol Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration. Authors - Xuemeng Shi View Article

ARPC2 Polyclonal antibody

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IHC staining of human breast cancer using 15058-1-AP

IF Staining of HeLa using 15058-1-AP

FC experiment of HeLa using 15058-1-AP

WB analysis of MCF-7 using 15058-1-AP

IP experiment of HeLa using 15058-1-AP

IHC staining of human normal colon using 15058-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Aberrant cortex contractions impact mammalian oocyte quality

Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein

α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.

WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.

The Proteome of BLOC-1 Genetic Defects Identifies the Arp2/3 Actin Polymerization Complex to Function Downstream of the Schizophrenia Susceptibility Factor Dysbindin at the Synapse.

Feedback-Driven Mechanisms Between Phosphorylated Caveolin-1 and Contractile Actin Assemblies Instruct Persistent Cell Migration.

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Flow Cytometry Perm Buffer (10X)