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  • Featured Product
  • KD/KO Validated

ARPC2 Polyclonal antibody, PBS Only

ARPC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15058-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 15058-1-AP (same clone as 15058-1-PBS)

    WB analysis using 15058-1-AP (same clone as 15058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

    IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

    IHC staining of human breast cancer using 15058-1-AP (same clone as 15058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARPC2 antibody included
  • IF Staining of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    IF Staining of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARPC2 antibody (15058-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    FC experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARPC2 (15058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 15058-1-AP (same clone as 15058-1-PBS)

    WB analysis of MCF-7 using 15058-1-AP (same clone as 15058-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15058-1-AP (ARPC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    IP experiment of HeLa using 15058-1-AP (same clone as 15058-1-PBS)

    IP result of anti-ARPC2 (IP:15058-1-AP, 4ug; Detection:15058-1-AP 1:6000) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 15058-1-AP (same clone as 15058-1-PBS)

    IHC staining of human colon using 15058-1-AP (same clone as 15058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15058-1-AP (ARPC2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15058-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ARPC2 antibody included
Planning an IHC experiment? We recommend our IHCeasy ARPC2 Ready-To-Use IHC Kit. ARPC2 primary antibody included.

产品信息

15058-1-PBS targets ARPC2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ARPC2 fusion protein Ag7041 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 2, 34kDa
Synonyms
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC000590
Gene Symbol ARPC2
Gene ID (NCBI) 10109
RRIDAB_2059787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15144
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C