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ARPC5L Polyclonal antibody, PBS Only

ARPC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22025-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 22025-1-AP (same clone as 22025-1-PBS)

    WB analysis using 22025-1-AP (same clone as 22025-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 22025-1-AP (same clone as 22025-1-PBS)

    IF Staining of MCF-7 using 22025-1-AP (same clone as 22025-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 22025-1-AP (same clone as 22025-1-PBS)

    IP experiment of mouse brain using 22025-1-AP (same clone as 22025-1-PBS)

    IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

产品信息

22025-1-PBS targets ARPC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ARPC5L fusion protein Ag16881 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin related protein 2/3 complex, subunit 5-like
Synonyms
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC000798
Gene Symbol ARPC5L
Gene ID (NCBI) 81873
RRIDAB_11182273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BPX5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.