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ACTRT3/ARPM1 Monoclonal antibody

ACTRT3/ARPM1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, rabbit

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B2B10

Cat No : 68372-1-Ig

Print datasheet

Synonyms

actin related protein M1, ARPM1

验证数据展示

  • WB analysis using 68372-1-Ig

    WB analysis using 68372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68372-1-Ig (ARPM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68372-1-Ig

    WB analysis using 68372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68372-1-Ig (ARPM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 68372-1-Ig

    IHC staining of human colon using 68372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 68372-1-Ig (ARPM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNCCIT cells, Rabbit testis, Rat testis, Human testis
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68372-1-Ig targets ACTRT3/ARPM1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, rabbit samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human, rat, rabbit
Immunogen ACTRT3/ARPM1 fusion protein Ag33162 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin related protein M1
Synonyms actin related protein M1, ARPM1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC007289
Gene Symbol ARPM1
Gene ID (NCBI) 84517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYD9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACTRT3/ARPM1 antibody 68372-1-IgDownload protocol
IHC protocol for ACTRT3/ARPM1 antibody 68372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols