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验证数据展示

  • WB analysis of mouse brain using 11829-1-AP

    WB analysis of mouse brain using 11829-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11829-1-AP (ARPP-21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 11829-1-AP

    IHC staining of mouse brain using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11829-1-AP

    IHC staining of mouse brain using 11829-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11829-1-AP (ARPP-21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARPP-21 Polyclonal antibody

ARPP-21 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11829-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

ARPP 21, ARPP21, ARPP-21, RCS, TARPP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11829-1-AP targets ARPP-21 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原 ARPP-21 fusion protein Ag2399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclic AMP-regulated phosphoprotein, 21 kD
别名 ARPP 21, ARPP21, ARPP-21, RCS, TARPP
计算分子量 89 kDa
观测分子量 86 kDa
GenBank蛋白编号BC017805
基因名称 ARPP-21
Gene ID (NCBI) 10777
RRIDAB_2877798
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBL0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARPP-21 antibody 11829-1-APDownload protocol
IHC protocol for ARPP-21 antibody 11829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Front Cell Dev Biol

PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.

Authors - Ye-Ya Tan
View Article
mouseWB

Nat Commun

The thymocyte-specific RNA-binding protein Arpp21 provides TCR repertoire diversity by binding to the 3'-UTR and promoting Rag1 mRNA expression

Authors - Meng Xu
View Article