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Beta Arrestin 2 Polyclonal antibody

Beta Arrestin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10171-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (3)

ARRB2, β-Arrestin 2, Beta-arrestin-2, BARR2, Arrestin beta-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue
Positive IHC detected inhuman tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, mouse Retina tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10171-1-AP targets Beta Arrestin 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, hamster, xenopus
免疫原 Beta Arrestin 2 fusion protein Ag0227 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arrestin, beta 2
别名 ARRB2, β-Arrestin 2, Beta-arrestin-2, BARR2, Arrestin beta-2
计算分子量 46 kDa
观测分子量 50 kDa
GenBank蛋白编号BC007427
基因名称 Beta Arrestin 2
Gene ID (NCBI) 409
RRIDAB_10644158
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP32121
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.

实验方案

Product Specific Protocols
WB protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IHC protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
IF protocol for Beta Arrestin 2 antibody 10171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.

Authors - Yuqing Wu
  • KO Validated
mouseWB

Biomaterials

Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure

Authors - Lingjun Tong
mouseWB

Sci Adv

Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome

Authors - Wei Wang
ratWB

Autophagy

Autophagy and post-ischemic conditioning in retinal ischemia.

Authors - Biji Mathew
human,mouseWB,IF,CoIP

J Pharm Anal

Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis

Authors - Jia-Jia Zhang
  • KD Validated
mouseIF

J Cell Biol

BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.

Authors - Fan Ye
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