验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue |
Positive IHC detected in | human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, mouse Retina tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10171-1-AP targets Beta Arrestin 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, hamster, xenopus |
免疫原 | Beta Arrestin 2 fusion protein Ag0227 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | arrestin, beta 2 |
别名 | ARRB2, β-Arrestin 2, Beta-arrestin-2, BARR2, Arrestin beta-2 |
计算分子量 | 46 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC007427 |
基因名称 | Beta Arrestin 2 |
Gene ID (NCBI) | 409 |
RRID | AB_10644158 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P32121 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
实验方案
Product Specific Protocols | |
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WB protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
IHC protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
IF protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
| ||
Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure | ||
Sci Adv Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome | ||
J Pharm Anal Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis
| ||
J Cell Biol BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. |