验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, RAW264.7, PC-12 cells, rat brain tissue |
| Positive IHC detected in | human tonsil tissue, human malignant melanoma tissue, human colon cancer tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, mouse Retina tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10171-1-AP targets Beta Arrestin 2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster, xenopus |
| Immunogen | Beta Arrestin 2 fusion protein Ag0227 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | arrestin, beta 2 |
| Synonyms | ARRB2, β-Arrestin 2, Beta-arrestin-2, BARR2, Arrestin beta-2 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC007427 |
| Gene Symbol | Beta Arrestin 2 |
| Gene ID (NCBI) | 409 |
| RRID | AB_10644158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P32121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| IHC protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| IF protocol for Beta Arrestin 2 antibody 10171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
| ||
Sci Adv Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome | ||
J Pharm Anal Monoclonal antibody targeting mu-opioid receptor attenuates morphine tolerance via enhancing morphine-induced receptor endocytosis
| ||
J Cell Biol BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone. | ||
Acta Pharmacol Sin Activation of GPR120 in podocytes ameliorates kidney fibrosis and inflammation in diabetic nephropathy. |
