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ARRDC1 Polyclonal antibody

ARRDC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21030-1-AP

Print datasheet

Synonyms

Arrestin domain-containing protein 1, arrestin domain containing 1, Alpha-arrestin 1

验证数据展示

  • WB analysis using 21030-1-AP

    WB analysis using 21030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21030-1-AP (ARRDC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293 exosomes, human plasma, human plasma exosomes

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21030-1-AP targets ARRDC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ARRDC1 fusion protein Ag15341 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arrestin domain containing 1
Synonyms Arrestin domain-containing protein 1, arrestin domain containing 1, Alpha-arrestin 1
Calculated Molecular Weight 433 aa, 46 kDa
Observed Molecular Weight46-50 kDa
GenBank Accession NumberBC032346
Gene Symbol ARRDC1
Gene ID (NCBI) 92714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N5I2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARRDC1, or Arrestin-Domain Containing Protein 1, is a protein that plays a significant role in the formation and release of extracellular vesicles, specifically a subtype known as ARMMs (Arrestin-Domain Containing Protein 1-mediated microvesicles). ARRDC1 is localized to the cytosolic side of the plasma membrane and recruits the ESCRT-I complex protein TSG101 to initiate outward membrane budding. This protein is also involved in the regulation of protein cargo and release of extracellular vesicles, including both exosomes and ectosomes. The absence of ARRDC1 can lead to changes in the protein cargo within these vesicles, indicating its importance in the sorting and packaging process.

实验方案

Product Specific Protocols
WB protocol for ARRDC1 antibody 21030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols