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ART5 Polyclonal antibody

ART5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11354-1-AP

Print datasheet

Synonyms

ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5

验证数据展示

  • WB analysis of mouse testis using 11354-1-AP

    WB analysis of mouse testis using 11354-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11354-1-AP (ART5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11354-1-AP

    IHC staining of human heart using 11354-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 11354-1-AP (ART5 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11354-1-AP targets ART5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ART5 fusion protein Ag1786 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ADP-ribosyltransferase 5
Synonyms ADP ribosyltransferase 5, ART5, Ecto ADP ribosyltransferase 5, Mono(ADP ribosyl)transferase 5
Calculated Molecular Weight 292 aa, 32 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC014577
Gene Symbol ART5
Gene ID (NCBI) 116969
RRIDAB_10734329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ART5 (Ecto-ADP-ribosyltransferase 5) belongs to a class of enzymes that catalyze the transfer of an ADP-ribose group from NAD to arginine residues on target proteins like G proteins. ART5 is a secreted protein. This antibody detects the 43 kDa ART5 protein in mouse liver and testis.

实验方案

Product Specific Protocols
WB protocol for ART5 antibody 11354-1-APDownload protocol
IHC protocol for ART5 antibody 11354-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols