ASAH1 Polyclonal antibody
ASAH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SH-SY5Y cells, SW480 cells, T-47D cells |
Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11274-1-AP targets ASAH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | ASAH1 fusion protein Ag1799 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
Synonyms | EC:3.5.1.-, ASAH, Acid ceramidase subunit beta, Acid ceramidase subunit alpha, Acid ceramidase |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 37-40 kDa |
GenBank Accession Number | BC016828 |
Gene Symbol | ASAH1 |
Gene ID (NCBI) | 427 |
RRID | AB_2058719 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13510 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ASAH1 antibody 11274-1-AP | Download protocol |
IHC protocol for ASAH1 antibody 11274-1-AP | Download protocol |
IF protocol for ASAH1 antibody 11274-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. | ||
Hum Mol Genet Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function. | ||
BMC Genomics Lipid metabolism-related genes are involved in the occurrence of asthma and regulate the immune microenvironment | ||
Int J Mol Sci Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation. | ||
J Dermatol Sci Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis. |