验证数据展示
经过测试的应用
| Positive WB detected in | rat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy ASAH1 Ready-To-Use IHC Kit. ASAH1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67092-1-Ig targets ASAH1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 文献引用反应性 | mouse |
| 免疫原 | ASAH1 fusion protein Ag28645 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
| 别名 | AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein |
| 计算分子量 | 44 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC016828 |
| 基因名称 | ASAH1 |
| Gene ID (NCBI) | 427 |
| RRID | AB_2882397 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13510 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| IHC protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| IF protocol for ASAH1 antibody 67092-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
