验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IP | See 2 publications below |
产品信息
17627-1-AP targets ASCC3 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | ASCC3 fusion protein Ag11848 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | activating signal cointegrator 1 complex subunit 3 |
| 别名 | Helicase, ATP binding 1, HELIC1, EC:3.6.4.12, ASC1p200, ASC-1 complex subunit p200 |
| 计算分子量 | 111 aa, 13 kDa, 251 kDa |
| 观测分子量 | 251 kDa |
| GenBank蛋白编号 | BC050681 |
| 基因名称 | ASCC3 |
| Gene ID (NCBI) | 10973 |
| RRID | AB_2059474 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N3C0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASCC3 (Activating Signal Cointegrator 1 Complex Subunit 3) is a multifunctional protein involved in various cellular processes, including transcriptional regulation, DNA repair, and ribosome quality control. It is a member of the ASCC (Activating Signal Cointegrator 1 Complex) family, which functions as a bridge between co-repressors and co-activators in transcriptional regulation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASCC3 antibody 17627-1-AP | Download protocol |
| IP protocol for ASCC3 antibody 17627-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex | ||
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Mol Cell Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress. | ||
Sci Rep Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells. | ||
Cell Metab Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response | ||
