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ASCL1 Polyclonal antibody

ASCL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23751-1-AP

Print datasheet

Synonyms

Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1

验证数据展示

  • IHC staining of human brain using 23751-1-AP

    IHC staining of human brain using 23751-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23751-1-AP (ASCL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 23751-1-AP

    WB analysis using 23751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23751-1-AP (ASCL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23751-1-AP targets ASCL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen ASCL1 fusion protein Ag19360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name achaete-scute complex homolog 1 (Drosophila)
Synonyms Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC003134
Gene Symbol ASCL1
Gene ID (NCBI) 429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDP50553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.

实验方案

Product Specific Protocols
WB protocol for ASCL1 antibody 23751-1-APDownload protocol
IHC protocol for ASCL1 antibody 23751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols