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验证数据展示

  • WB analysis of MKN-45 using 21368-1-AP

    WB analysis of MKN-45 using 21368-1-AP

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 21368-1-AP (ASCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 21368-1-AP

    IHC staining of human stomach cancer using 21368-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21368-1-AP (ASCL2 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ASCL2 Polyclonal antibody

ASCL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21368-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Class A basic helix-loop-helix protein 45, bHLHa45, ASH-2, ASH2, Achaete-scute homolog 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMKN-45 cells, Caco-2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

21368-1-AP targets ASCL2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 ASCL2 fusion protein Ag15839 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 achaete-scute complex homolog 2 (Drosophila)
别名 Class A basic helix-loop-helix protein 45, bHLHa45, ASH-2, ASH2, Achaete-scute homolog 2
计算分子量 193 aa, 20 kDa
观测分子量20 kDa
GenBank蛋白编号BC057801
基因名称 ASCL2
Gene ID (NCBI) 430
RRIDAB_2935452
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99929
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Achaete-scute complex homolog 2 (Drosophila), also known as ASCL2, is an imprinted human gene. This gene is involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system, particularly important during implantation of the developing embryo, specifically in placental development and neuronal precursor determination. ASCL2 has been reported involved in tumor progression, being upregulated in colorectal tumors, and involvement in metastasis could be attributed to ASCL2-promoted cellular self-renewal as opposed to cellular differentiation (PMID: 30096149 16568095).

实验方案

Product Specific Protocols
WB protocol for ASCL2 antibody 21368-1-APDownload protocol
IHC protocol for ASCL2 antibody 21368-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

tRNA-derived RNA fragment, tRF-18-8R6546D2, promotes pancreatic adenocarcinoma progression by directly targeting ASCL2

Authors - Sihua Lan
View Article