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ASF/SF2 Monoclonal antibody, PBS Only

ASF/SF2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6C5

Cat no : 66671-1-PBS

Print datasheet

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

验证数据展示

  • WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

    IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

    IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SRSF1 antibody included
  • IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SRSF1 Ready-To-Use IHC Kit. SRSF1 primary antibody included.

产品信息

66671-1-PBS targets ASF/SF2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ASF/SF2 fusion protein Ag3626 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 1
Synonyms Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 32 kDa, 28 kDa, 22 kDa
GenBank Accession NumberBC010264
Gene Symbol ASF/SF2
Gene ID (NCBI) 6426
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ07955
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.