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ASF/SF2 Monoclonal antibody, PBS Only

ASF/SF2 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1A6C5

Cat No : 66671-1-PBS

Print datasheet

Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

验证数据展示

  • WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    WB analysis using 66671-1-Ig (same clone as 66671-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

    IP experiment of HeLa using 66671-1-Ig (same clone as 66671-1-PBS)

    IP result of anti-ASF/SF2 (IP:66671-1-Ig, 5ug; Detection:66671-1-Ig 1:5000) with HeLa cells lysate 2200 ug. This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    IHC staining of human ovary tumor using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66671-1-Ig (ASF/SF2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SRSF1 antibody included
  • IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    IF Staining of HepG2 using 66671-1-Ig (same clone as 66671-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ASF/SF2 antibody (66671-1-Ig, Clone: 1A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66671-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SRSF1 Ready-To-Use IHC Kit. SRSF1 primary antibody included.

产品信息

66671-1-PBS targets ASF/SF2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen ASF/SF2 fusion protein Ag3626 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 1
Synonyms Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 32 kDa, 28 kDa, 22 kDa
GenBank Accession NumberBC010264
Gene Symbol SFRS1
Gene ID (NCBI) 6426
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ07955
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.