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验证数据展示

  • WB analysis using 22259-1-AP

    WB analysis using 22259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 22259-1-AP

    IF Staining of A431 using 22259-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ASF1A-specific antibody (22259-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO Validated

ASF1A-specific Polyclonal antibody

ASF1A-specific Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22259-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22259-1-AP targets ASF1A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ASF1A-specific fusion protein Ag17764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Synonyms ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
RRIDAB_2879052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.

实验方案

Product Specific Protocols
WB protocol for ASF1A-specific antibody 22259-1-APDownload protocol
IF protocol for ASF1A-specific antibody 22259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.

Authors - Sumin Feng
  • KD Validated
View Article