验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22259-1-AP targets ASF1A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | ASF1A-specific fusion protein Ag17764 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ASF1 anti-silencing function 1 homolog A (S. cerevisiae) |
别名 | ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A |
计算分子量 | 204 aa, 23 kDa |
观测分子量 | 20-23 kDa |
GenBank蛋白编号 | BC010878 |
基因名称 | ASF1A |
Gene ID (NCBI) | 25842 |
RRID | AB_2879052 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y294 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.
实验方案
Product Specific Protocols | |
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WB protocol for ASF1A-specific antibody 22259-1-AP | Download protocol |
IF protocol for ASF1A-specific antibody 22259-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |