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ASF1A Monoclonal antibody, PBS Only

ASF1A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D4A6

Cat No : 66884-1-PBS

Print datasheet

Synonyms

ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

验证数据展示

  • WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

    WB analysis of Jurkat using 66884-1-Ig (same clone as 66884-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66884-1-Ig (ASF1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

    IF Staining of HepG2 using 66884-1-Ig (same clone as 66884-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66884-1-Ig (ASF1A antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66884-1-PBS in a different storage buffer formulation.

产品信息

66884-1-PBS targets ASF1A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen ASF1A fusion protein Ag1233 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
Synonyms ASF1A, CCG1 interacting factor A, CGI 98, CIA, DKFZp547E2110, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight 21-23 kDa
GenBank Accession NumberBC010878
Gene Symbol ASF1A
Gene ID (NCBI) 25842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y294
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Anti-silencing function 1, S.cerevisiae, homolog of A(ASF1A) is a histone chaperone that has a crucial role in the remodeling of chromatin structure during replication, DNA repair, and cellular senescence. It promotes chromation assembly in replication-dependent manner by cooperation with chromation assembly factor1(CAF-1), and cooperates with HIRA in replication-independent manner. It's required forthe fomation of senescence-associated heterochromation foci(SAHF) and efficient senescence-associated cell cycle exit.