验证数据展示
经过测试的应用
Positive WB detected in | mouse thymus tissue, HeLa cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11011-1-AP targets ASF1A/B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | ASF1A/B fusion protein Ag1477 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ASF1 anti-silencing function 1 homolog B (S. cerevisiae) |
别名 | ASF1B, Anti-silencing function protein 1 homolog B, hAsf1, CIA-II, CIA II |
计算分子量 | 202 aa, 22 kDa |
观测分子量 | 22 kDa |
GenBank蛋白编号 | BC010014 |
基因名称 | ASF1B |
Gene ID (NCBI) | 55723 |
RRID | AB_2059670 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NVP2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody reacts with the ASF1B and ASF1A protein.
实验方案
Product Specific Protocols | |
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WB protocol for ASF1A/B antibody 11011-1-AP | Download protocol |
IHC protocol for ASF1A/B antibody 11011-1-AP | Download protocol |
IF protocol for ASF1A/B antibody 11011-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |