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验证数据展示

  • WB analysis using 22258-1-AP (same clone as 22258-1-PBS)

    WB analysis using 22258-1-AP (same clone as 22258-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22258-1-AP (ASF1B-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    IHC staining of human lymphoma using 22258-1-AP (same clone as 22258-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22258-1-AP (ASF1B-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASF1B antibody included
  • IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22258-1-AP (ASF1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    IF Staining of HeLa using 22258-1-AP (same clone as 22258-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF1B-specific antibody (22258-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22258-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ASF1B-specific Polyclonal antibody, PBS Only

ASF1B-specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22258-1-PBS

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ASF1B, ASF1A/B, CIA-II, CIA II, CCG1-interacting factor A-II

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASF1B Ready-To-Use IHC Kit. ASF1B primary antibody included.

产品信息

22258-1-PBS targets ASF1B-specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen ASF1B-specific fusion protein Ag17763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ASF1 anti-silencing function 1 homolog B (S. cerevisiae)
Synonyms ASF1B, ASF1A/B, CIA-II, CIA II, CCG1-interacting factor A-II
Calculated Molecular Weight 202 aa, 22 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC010014
Gene Symbol ASF1B
Gene ID (NCBI) 55723
RRIDAB_2879051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVP2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASF1B is a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. This antibody specifically reacts with the 19kd ASF1B protein.