ASGR1 Recombinant antibody

ASGR1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83691-3-RR

产品说明书

CloneNo. 240672C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHepG2 cells, HuH-7 cells, human liver tissue, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat liver tissue
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83691-3-RR targets ASGR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 asialoglycoprotein receptor 1
别名 asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
计算分子量33 kDa
观测分子量 42-46 kDa
GenBank蛋白编号NM_001671.4
基因名称 ASGR1
Gene ID (NCBI) 432
RRIDAB_3671295
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07306-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR1 antibody 83691-3-RRDownload protocol
IHC protocol for ASGR1 antibody 83691-3-RRDownload protocol
IF protocol for ASGR1 antibody 83691-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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