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验证数据展示

  • WB analysis of human liver using 11501-2-AP

    WB analysis of human liver using 11501-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 11501-2-AP

    FC experiment of HepG2 using 11501-2-AP

    1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

  • IP experiment of L02 using 11501-2-AP

    IP experiment of L02 using 11501-2-AP

    IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug.

  • IHC staining of human liver using 11501-2-AP

    IHC staining of human liver using 11501-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ASGR2 antibody included
  • IHC staining of human liver using 11501-2-AP

    IHC staining of human liver using 11501-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ASGR2 antibody included

ASGR2 Polyclonal antibody

ASGR2 Polyclonal Antibody for WB, IHC, FC, IP, ELISA
Cat No. 11501-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC, IP, ELISA

别名

CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR2 Ready-To-Use IHC Kit. ASGR2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11501-2-AP targets ASGR2 in WB, IHC, FC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC, IP, ELISA Application Description
经测试反应性 human
免疫原 ASGR2 fusion protein Ag2037 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asialoglycoprotein receptor 2
别名 CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC017251
基因名称 ASGR2
Gene ID (NCBI) 433
RRIDAB_2059814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07307
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR2 antibody 11501-2-APDownload protocol
IHC protocol for ASGR2 antibody 11501-2-APDownload protocol
IP protocol for ASGR2 antibody 11501-2-APDownload protocol
FC protocol for ASGR2 antibody 11501-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols