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ASGR2 Polyclonal antibody

ASGR2 Polyclonal Antibody for WB, IHC, FC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11501-2-AP

Print datasheet

Synonyms

CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2

验证数据展示

  • WB analysis of human liver using 11501-2-AP

    WB analysis of human liver using 11501-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11501-2-AP (ASGR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 11501-2-AP

    FC experiment of HepG2 using 11501-2-AP

    1x10^6 HepG2 cells were surface stained with 0.25 ug ASGR2 Polyclonal antibody (11501-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

  • IP experiment of L02 using 11501-2-AP

    IP experiment of L02 using 11501-2-AP

    IP result of anti-ASGR2 (IP:11501-2-AP, 4ug; Detection:11501-2-AP 1:300) with L02 cells lysate 1800ug.

  • IHC staining of human liver using 11501-2-AP

    IHC staining of human liver using 11501-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ASGR2 antibody included
  • IHC staining of human liver using 11501-2-AP

    IHC staining of human liver using 11501-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11501-2-AP (ASGR2 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ASGR2 antibody included

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ASGR2 Ready-To-Use IHC Kit. ASGR2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11501-2-AP targets ASGR2 in WB, IHC, FC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC, IP, ELISA Application Description
Tested Reactivity human
Immunogen ASGR2 fusion protein Ag2037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asialoglycoprotein receptor 2
Synonyms CLEC4H2, asialoglycoprotein receptor 2, ASGP-R 2, ASGPR 2, ASGP R 2
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC017251
Gene Symbol ASGR2
Gene ID (NCBI) 433
RRIDAB_2059814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07307
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR2 antibody 11501-2-APDownload protocol
IHC protocol for ASGR2 antibody 11501-2-APDownload protocol
IP protocol for ASGR2 antibody 11501-2-APDownload protocol
FC protocol for ASGR2 antibody 11501-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols