ASK1 Polyclonal antibody

ASK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28201-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (3)

应用

WB, IHC, ELISA and More (1)

ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A431 cells, BxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28201-1-AP targets ASK1 in WB, IHC, IP, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 Human, Mouse
文献引用反应性human, mouse, rat
免疫原 ASK1 fusion protein Ag28092 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 5
别名 ASK 1, ASK1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, MEK kinase 5, MEKK 5, MEKK5
计算分子量 155 kDa
观测分子量 155 kDa
GenBank蛋白编号BC054503
基因名称 ASK1
Gene ID (NCBI) 4217
RRIDAB_2782957
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99683
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASK1(Apoptosis signal-regulating kinase 1) is also named as MAP3K5, MAPKKK5, MEKK5 and belongs to the MAP kinase kinase kinase subfamily. It is an evolutionarily conserved mitogen activated protein 3-kinase that activates both Jnk and p38 mitogen-activated protein kinases. ASK1 is activated in response to various cytotoxic stresses including TNF, Fas and reactive oxygen species (ROS) such as H2O2, and activates c-Jun NH 2-terminal kinase (JNK) and p38. 28201-1-AP antibody detects the 155 kDa band in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for ASK1 antibody 28201-1-APDownload protocol
IHC protocol for ASK1 antibody 28201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Redox Biol

FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response

Authors - Zhe Wang
ratWB

Oxid Med Cell Longev

Melatonin Alleviates Neuroinflammation and Metabolic Disorder in DSS-Induced Depression Rats.

Authors - Wei-Jie Lv
mouseWB

FASEB J

GS-4997 halts the progression of tubulointerstitial injury in lupus nephritis

Authors - Zheng Xiao
ratWB

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang
mouseWB

Food Funct

L-Theanine mediates the p38MAPK signaling pathway to alleviate heat-induced oxidative stress and inflammation in mice.

Authors - Kehong Liu
ratWB

Life Sci

The IRE1 signaling pathway is involved in the protective effect of low-dose LPS on myocardial ischemia-reperfusion injury.

Authors - Ting Wu
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