- Featured Product
- KD/KO Validated
ASNA1 Polyclonal antibody
ASNA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, HeLa cells, human heart tissue, mouse brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 3 publications below |
WB | See 6 publications below |
产品信息
15450-1-AP targets ASNA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | ASNA1 fusion protein Ag7713 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) |
Synonyms | TRC40, EC:3.6.-.-, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase |
Calculated Molecular Weight | 38.7 kDa |
Observed Molecular Weight | 37-41 kDa |
GenBank Accession Number | BC002651 |
Gene Symbol | ASNA1 |
Gene ID (NCBI) | 439 |
RRID | AB_2060110 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43681 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for ASNA1 antibody 15450-1-AP | Download protocol |
IHC protocol for ASNA1 antibody 15450-1-AP | Download protocol |
IF protocol for ASNA1 antibody 15450-1-AP | Download protocol |
IP protocol for ASNA1 antibody 15450-1-AP | Download protocol |
FC protocol for ASNA1 antibody 15450-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Molecular machinery for insertion of tail-anchored membrane proteins into the endoplasmic reticulum membrane in mammalian cells. | ||
J Cell Sci Emery-Dreifuss muscular dystrophy mutations impair TRC40-mediated targeting of emerin to the inner nuclear membrane.
| ||
J Cell Sci An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1.
| ||
J Biol Chem ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence | ||
FEBS Lett Human SND2 mediates ER targeting of GPI-anchored proteins with low hydrophobic GPI attachment signals. |