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验证数据展示

  • WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

    IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

    IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

    IHC staining of human lung cancer using 66346-1-Ig (same clone as 66346-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66346-1-Ig (ASNA1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    WB analysis using 66346-1-Ig (same clone as 66346-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66346-1-Ig (ASNA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

    IF Staining of HEK-293 using 66346-1-Ig (same clone as 66346-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ASNA1 antibody (66346-1-Ig, Clone: 1B7C11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66346-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50801-1

    Cytometric bead array standard curve of MP50801-1

    Cytometric bead array standard curve of MP50801-1, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-2-PBS. Standard:Ag7936. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50801-2

    Cytometric bead array standard curve of MP50801-2

    Cytometric bead array standard curve of MP50801-2, ASNA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66346-1-PBS. Detection antibody: 66346-3-PBS. Standard:Ag7936. Range: 0.391-100 ng/mL.

ASNA1 Monoclonal antibody, PBS Only (Capture)

ASNA1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66346-1-PBS

产品说明书

CloneNo. 1B7C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, chicken

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66346-1-PBS targets ASNA1 as part of a matched antibody pair:

MP50801-1: 66346-1-PBS capture and 66346-2-PBS detection (validated in Cytometric bead array)

MP50801-2: 66346-1-PBS capture and 66346-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, chicken
免疫原 ASNA1 fusion protein Ag7936 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
别名 TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase
计算分子量 38.7 kDa
观测分子量 37-41 kDa
GenBank蛋白编号BC002651
基因名称 ASNA1
Gene ID (NCBI) 439
RRIDAB_2881726
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43681
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.