验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue |
Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13244-1-AP targets ASPA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | ASPA fusion protein Ag4029 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | aspartoacylase (Canavan disease) |
Synonyms | ACY 2, ACY2, Aminoacylase 2, ASP, ASPA, Aspartoacylase |
Calculated Molecular Weight | 313 aa, 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC029128 |
Gene Symbol | ASPA |
Gene ID (NCBI) | 443 |
RRID | AB_2274358 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P45381 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASPA(Aspartoacylase) also called aminoacylase-2, is an enzyme that hydrolyzes N-acetyl-L-aspartic acid (NAA) to aspartate and acetate.Human ASPA encodes a deduced 313-amino acid protein with a molecular mass of 36 KDa. It shares 92% sequence identity with the bovine protein and contains 1 potential N-glycosylation site and 5 phosphorylation sites.Defects in ASPA are the cause of Canavan disease (CAND).This protein can exsit as a homodimer(18293939).
实验方案
Product Specific Protocols | |
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WB protocol for ASPA antibody 13244-1-AP | Download protocol |
IHC protocol for ASPA antibody 13244-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mil Med Res Aspartoacylase suppresses prostate cancer progression by blocking LYN activation | ||
Elife Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. | ||
Glia Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome | ||
J Neurosci Suppressing N-Acetyl-l-Aspartate Synthesis Prevents Loss of Neurons in a Murine Model of Canavan Leukodystrophy.
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Front Pharmacol Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress. |