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ASUN Recombinant antibody

ASUN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241586E9

Cat no : 84252-4-RR

Print datasheet

Synonyms

INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11

验证数据展示

  • WB analysis using 84252-4-RR

    WB analysis using 84252-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84252-4-RR (C12orf11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84252-4-RR

    IF Staining of HeLa using 84252-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASUN antibody (84252-4-RR, Clone: 241586E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84252-4-RR

    Affinity and Kinetic Characterization of 84252-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84252-4-RR against Human ASUN were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ASUN Ready-To-Use IHC Kit. ASUN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84252-4-RR is ASUN Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ASUN fusion protein Ag13727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 12 open reading frame 11
Synonyms INTS13, Integrator complex subunit 13, Germ cell tumor 1, GCT1, C12orf11
Calculated Molecular Weight 706 aa, 80 kDa
Observed Molecular Weight70-80 kDa
GenBank Accession NumberBC003081
Gene Symbol ASUN
Gene ID (NCBI) 55726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NVM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asunder (ASUN) is a conserved protein that has been reported as a critical regulator of dynein localization during Drosophila spermatogenesis (PMID: 19357193). Mammalian and Drosophila ASUN proteins revealed that they are 43% identical and 64% similar. Human ASUN (also known as GCT1 or C12ORF11) promotes dynein recruitment and centrosomal tethering to the nucleus at mitotic entry (PMID: 23097494).

实验方案

Product Specific Protocols
WB protocol for ASUN antibody 84252-4-RRDownload protocol
IF protocol for ASUN antibody 84252-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols