• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ASXL1 Recombinant antibody

ASXL1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241563A5

Cat no : 84630-1-RR

Print datasheet

Synonyms

Polycomb group protein ASXL1, KIAA0978, Additional sex combs-like protein 1, 241563A5

验证数据展示

  • WB analysis using 84630-1-RR

    WB analysis using 84630-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84630-1-RR (ASXL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84630-1-RR

    Affinity and Kinetic Characterization of 84630-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84630-1-RR against Human ASXL1 were performed. The affinity constant is 39.3 nM.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, HL-60 cells, COLO 320 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84630-1-RR targets ASXL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ASXL1 fusion protein Ag10290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name additional sex combs like 1 (Drosophila)
Synonyms Polycomb group protein ASXL1, KIAA0978, Additional sex combs-like protein 1, 241563A5
Calculated Molecular Weight84aa,10 kDa; 1541aa,165 kDa
Observed Molecular Weight200-220 kDa
GenBank Accession NumberBC064984
Gene Symbol ASXL1
Gene ID (NCBI) 171023
RRIDAB_3672092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Additional sex combs-like 1 (ASXL1) is a member of the ASXL family, which is involved in epigenetic regulation. It is one of the mammalian Asx homologs. Mutations in the ASXL1 gene are frequently found in myeloid neoplasms, breast cancers, and prostate cancers (PMID: 30013160; PMID: 22436456; PMID: 26470845). In patients with myeloid malignancies, ASXL1 mutations are mostly heterozygous frameshift or nonsense mutations that result in C-terminal truncation (PMID: 30982744; PMID: 26095772; PMID: 32683582; PMID: 29643185).

实验方案

Product Specific Protocols
WB protocol for ASXL1 antibody 84630-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols