验证数据展示
经过测试的应用
| Positive WB detected in | MAF cells |
| Positive IP detected in | NCCIT cell |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16610-1-AP targets ATAD3A/B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ATAD3A/B fusion protein Ag9836 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ATPase family, AAA domain containing 3B |
| Synonyms | AAA TOB3, ATAD3A, ATAD3B, KIAA1273, TOB3 |
| Calculated Molecular Weight | 72 kDa |
| Observed Molecular Weight | 68 kDa |
| GenBank Accession Number | BC002542 |
| Gene Symbol | ATAD3B |
| Gene ID (NCBI) | 83858 |
| RRID | AB_2878288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T9A4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATAD3 (ATPase family AAA Domain-containing protein 3) is a mitochondrial membrane bound ATPase whose function has not yet been discovered but its role is essential for embryonic development. The analysis of the cellular properties of ATAD3A and ATAD3B in different human cancer cell lines shows on the contrary that they can present anti-proliferative and chemoresistant properties. This antibody can detect both ATAD3A and ATAD3B.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| IP protocol for ATAD3A/B antibody 16610-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
EMBO J ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.
| ||
Oxid Med Cell Longev miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.
| ||
Int J Mol Sci Analysis of Radiation Toxicity in Mammalian Cells Stably Transduced with Mitochondrial Stat3 | ||
J Cell Sci ATAD3 controls mitochondrial cristae structure, influencing mtDNA replication and cholesterol levels in muscle.
|