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ATAD4 / PRR15L Polyclonal antibody

ATAD4 / PRR15L Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25693-1-AP

Print datasheet

Synonyms

ATAD4, Protein ATAD4, PRR15L

验证数据展示

  • IHC staining of mouse kidney using 25693-1-AP

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 25693-1-AP

    IHC staining of mouse kidney using 25693-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25693-1-AP (ATAD4 / PRR15L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 25693-1-AP

    IF Staining of A431 using 25693-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATAD4 / PRR15L antibody (25693-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25693-1-AP targets ATAD4 / PRR15L in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATAD4 / PRR15L fusion protein Ag22500 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase family, AAA domain containing 4
Synonyms ATAD4, Protein ATAD4, PRR15L
Calculated Molecular Weight 103 aa, 12 kDa
GenBank Accession NumberBC002865
Gene Symbol ATAD4
Gene ID (NCBI) 79170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATAD4 (ATPase family AAA domain-containing protein 4), also known as PRR15L (Proline Rich 15 Like), and its function has not yet been discovered.

实验方案

Product Specific Protocols
IHC protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
IF protocol for ATAD4 / PRR15L antibody 25693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols