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ATE1 Polyclonal antibody

ATE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13973-1-AP

Print datasheet

Synonyms

arginyltransferase 1, ATE1, R transferase 1

验证数据展示

  • WB analysis using 13973-1-AP

    WB analysis using 13973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13973-1-AP (ATE1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13973-1-AP

    WB analysis of HeLa using 13973-1-AP

    WB result of ATE1 antibody (13973-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATE1 transfected HeLa cells.

经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13973-1-AP targets ATE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATE1 fusion protein Ag5006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name arginyltransferase 1
Synonyms arginyltransferase 1, ATE1, R transferase 1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59-65 kDa
GenBank Accession NumberBC022026
Gene Symbol ATE1
Gene ID (NCBI) 11101
RRIDAB_2060763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginyltransferases (ATE1s) are eukaryotic enzymes that catalyze the non-ribosomal, post-translational addition of the amino acid arginine to an acceptor protein. ATE1 plays critical roles in many biological functions including cardiovascular development, angiogenesis, adipogenesis, muscle contraction, and metastasis of cancer.

实验方案

Product Specific Protocols
WB protocol for ATE1 antibody 13973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols