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ATF1 Polyclonal antibody
ATF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, A431 cells, human brain tissue |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 4 publications below |
WB | See 9 publications below |
IHC | See 1 publications below |
产品信息
11946-1-AP targets ATF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | ATF1 fusion protein Ag2548 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | activating transcription factor 1 |
Synonyms | TREB36, Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1 |
Calculated Molecular Weight | 271 aa, 29 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC029619 |
Gene Symbol | ATF1 |
Gene ID (NCBI) | 466 |
RRID | AB_2060777 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P18846 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATF1 antibody 11946-1-AP | Download protocol |
IF protocol for ATF1 antibody 11946-1-AP | Download protocol |
IP protocol for ATF1 antibody 11946-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Am J Hum Genet Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.
| ||
Mol Ther Nucleic Acids Mbd2 deficiency alleviates retinal cell apoptosisvia the miR-345-5p/Atf1 axis in high glucoseinjury and streptozotocin-induced diabetic mice. | ||
Front Cell Dev Biol CREB1 and ATF1 Negatively Regulate Glutathione Biosynthesis Sensitizing Cells to Oxidative Stress.
| ||
J Biol Chem The mRNA levels of heat shock factor 1 are regulated by thermogenic signals via the cAMP-dependent transcription factor ATF3. | ||
Cells Perilipin 5 Protects against Cellular Oxidative Stress by Enhancing Mitochondrial Function in HepG2 Cells. |