ATF2 Polyclonal antibody
ATF2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, mouse brain tissue, HeLa cells, K-562 cells |
Positive IP detected in | mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11908-1-AP targets ATF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Immunogen | ATF2 fusion protein Ag2581 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | activating transcription factor 2 |
Synonyms | CREB-2, CREB2, CREB 2, cAMP-responsive element-binding protein 2, cAMP-dependent transcription factor ATF-2 |
Calculated Molecular Weight | 209 aa, 23 kDa |
Observed Molecular Weight | 60 kDa, 64 kDa |
GenBank Accession Number | BC026175 |
Gene Symbol | ATF2 |
Gene ID (NCBI) | 1386 |
RRID | AB_10695892 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P15336 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ATF2 antibody 11908-1-AP | Download protocol |
IF protocol for ATF2 antibody 11908-1-AP | Download protocol |
IP protocol for ATF2 antibody 11908-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mar Drugs 7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway. | ||
Transl Cancer Res Annexin A7 and JNK knockdown suppress the lymphatic metastasis potential of hepatocellular carcinoma cells: Possible contributions of ATF2 and sequence-related lncRNA NONMMUT114121.1. | ||
Korean J Physiol Pharmacol The WNT/Ca2+ pathway promotes atrial natriuretic peptide secretion by activating protein kinase C/transforming growth factor-β activated kinase 1/activating transcription factor 2 signaling in isolated beating rat atria | ||
J Ethnopharmacol Isoastragaloside I attenuates cholestatic liver diseases by ameliorating liver injury, regulating bile acid metabolism and restoring intestinal barrier |