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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

ATF5 Monoclonal antibody, PBS Only

ATF5 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67066-1-PBS

产品说明书

CloneNo. 1E5B6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, Indirect ELISA

别名

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67066-1-PBS targets ATF5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human
免疫原 ATF5 fusion protein Ag7214 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 activating transcription factor 5
别名 ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx
计算分子量 31 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC005174
基因名称 ATF5
Gene ID (NCBI) 22809
RRIDAB_2882376
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2D1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).