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ATF5 Monoclonal antibody, PBS Only

ATF5 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5B6

Cat no : 67066-1-PBS

Print datasheet

Synonyms

ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx

验证数据展示

  • WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    WB analysis using 67066-1-Ig (same clone as 67066-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67066-1-Ig (ATF5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67066-1-PBS in a different storage buffer formulation.

产品信息

67066-1-PBS targets ATF5 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ATF5 fusion protein Ag7214 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name activating transcription factor 5
Synonyms ATF5, ATFX, FLJ34666, HMFN0395, Transcription factor ATFx
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC005174
Gene Symbol ATF5
Gene ID (NCBI) 22809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2D1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATF5, also named as Activating transcription factor 5, is a 282 amino acid protein, which belongs to the bZIP family. ATF5 is Widely expressed with higher expression levels in liver. ATF5 is actively transported to the centrosome and accumulated in the pericentriolar material (PCM) during G1 to M phase via a microtubule-dependent mechanism. During late telophase and cytokinesis, it translocates from the centrosome to the midbody (PMID: 26213385) . ATF5 as a transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically ATF5 is involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631).