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  • Featured Product
  • KD/KO Validated

ATF6 Monoclonal antibody, PBS Only

ATF6 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3B7E4

Cat no : 66563-1-PBS

Print datasheet

Synonyms

ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6

验证数据展示

  • WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

    WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

    IHC staining of human cervical cancer using 66563-1-Ig (same clone as 66563-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66563-1-Ig (ATF6 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF6 antibody included
  • WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

    WB analysis using 66563-1-Ig (same clone as 66563-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66563-1-Ig (ATF6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66563-1-Ig (same clone as 66563-1-PBS)

    WB analysis of HEK-293 using 66563-1-Ig (same clone as 66563-1-PBS)

    WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HEK-293 cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    WB analysis of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    WB result of ATF6 antibody (66563-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATF6 transfected HeLa cells. This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    IF Staining of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATF6 antibody (66563-1-Ig, Clone: 3B7E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    FC experiment of HeLa using 66563-1-Ig (same clone as 66563-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ATF6 (66563-1-Ig, Clone:3B7E4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66563-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ATF6 Ready-To-Use IHC Kit. ATF6 primary antibody included.

产品信息

66563-1-PBS targets ATF6 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ATF6 fusion protein Ag21456 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activating transcription factor 6
Synonyms ATF6-alpha, ATF6A, ATF6 alpha, ATF 6, activating transcription factor 6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC014969
Gene Symbol ATF6
Gene ID (NCBI) 22926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP18850
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activating transcription factor 6 (ATF6) is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor.During unfolded protein response an approximative 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. The fully glycosylated form of ATF6, a 670 amino acid protein, exhibits an electrophoretic mobility of ~90 kDa in denaturing SDS-gels, in part because of the glycosylated modifications. ATF6 has 3 consensus sites for N-linked glycosylation and exists constitutively as a glycosylated protein. Differentially glycosylated ATF6 forms may result from mutations or experimental treatment (PMID:15804611) (PMID:14699159). The antibody recognizes cleaved and fully glycosylated forms of ATF6.