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ATF7 Polyclonal antibody

ATF7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29770-1-AP

Print datasheet

Synonyms

ATF7, ATFA, Transcription factor ATF A

验证数据展示

  • WB analysis using 29770-1-AP

    WB analysis using 29770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29770-1-AP (ATF7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 29770-1-AP

    IHC staining of mouse lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of mouse lung using 29770-1-AP

    IHC staining of mouse lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human lung using 29770-1-AP

    IHC staining of human lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human lung using 29770-1-AP

    IHC staining of human lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of human urothelial carcinoma using 29770-1-AP

    IHC staining of human urothelial carcinoma using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of rat heart using 29770-1-AP

    IHC staining of rat heart using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included
  • IHC staining of rat lung using 29770-1-AP

    IHC staining of rat lung using 29770-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 29770-1-AP (ATF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATF7 antibody included

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung tissue, mouse lung tissue, human urothelial carcinoma tissue, mouse heart tissue, rat heart tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATF7 Ready-To-Use IHC Kit. ATF7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29770-1-AP targets ATF7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ATF7 fusion protein Ag30882 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activating transcription factor 7
Synonyms ATF7, ATFA, Transcription factor ATF A
Calculated Molecular Weight 53 kDa
Observed Molecular Weight52-60 kDa
GenBank Accession NumberNM_001366555
Gene Symbol ATF7
Gene ID (NCBI) 11016
RRIDAB_3086157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ATF7 antibody 29770-1-APDownload protocol
IHC protocol for ATF7 antibody 29770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols