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验证数据展示

  • IHC staining of human breast cancer using 14699-1-AP

    IHC staining of human breast cancer using 14699-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14699-1-AP

    IHC staining of human breast cancer using 14699-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14699-1-AP

    IHC staining of human ovary tumor using 14699-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14699-1-AP (ATF7IP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ATF7IP Polyclonal antibody

ATF7IP Polyclonal Antibody for IHC, ELISA
Cat No. 14699-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA and More (2)

别名

ATF-interacting protein, ATFa-associated modulator, ATF7-interacting protein, ATF7, ATF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue, human breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14699-1-AP targets ATF7IP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ATF7IP fusion protein Ag6341 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activating transcription factor 7 interacting protein
别名 ATF-interacting protein, ATFa-associated modulator, ATF7-interacting protein, ATF7, ATF
计算分子量 137 kDa
GenBank蛋白编号BC063855
基因名称 ATF7IP
Gene ID (NCBI) 55729
RRIDAB_2878074
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6VMQ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATF7IP (Activating Transcription Factor 7 Interacting Protein), also known as MCAF1 (MBD1-containing chromatin-associated factor 1), is a multifunctional scaffold protein that plays a pivotal role in epigenetic regulation and transcriptional control. Functionally, ATF7IP acts as an adaptor that recruits the histone methyltransferase SETDB1 to specific genomic loci, facilitating the deposition of the heterochromatin mark H3K9me3 and silencing target genes, including retrotransposons such as LINE1. This activity is essential for maintaining genomic stability by suppressing transposable element mobilization and preventing DNA damage.

实验方案

Product Specific Protocols
IHC protocol for ATF7IP antibody 14699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Hum Mol Genet

Mechanism suppressing H3K9 trimethylation in pluripotent stem cells and its demise by polyQ-expanded huntingtin mutations.

Authors - Dilber Irmak
  • KD Validated
View Article