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ATG10 Polyclonal antibody

ATG10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13406-1-AP

Print datasheet

Synonyms

EC:2.3.2.-, Autophagy-related protein 10, Autophagy related protein 10, APG10-like, APG10L

验证数据展示

  • WB analysis using 13406-1-AP

    WB analysis using 13406-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13406-1-AP (ATG10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13406-1-AP targets ATG10 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ATG10 fusion protein Ag4052 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG10 autophagy related 10 homolog (S. cerevisiae)
Synonyms EC:2.3.2.-, Autophagy-related protein 10, Autophagy related protein 10, APG10-like, APG10L
Calculated Molecular Weight 220 aa, 25 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC029268
Gene Symbol ATG10
Gene ID (NCBI) 83734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0Y0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG10, also named as APG10L, is an E2-like enzyme involved in autophagy. It catalyzes the conjugation reaction between Atg12 and Atg5. ATG10 plays a role in adenovirus-mediated cell lysis.

实验方案

Product Specific Protocols
WB protocol for ATG10 antibody 13406-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols