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  • Featured Product
  • KD/KO Validated

ATG12 Polyclonal antibody, PBS Only

ATG12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11264-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

    WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

    IHC staining of human colon cancer using 11264-1-AP (same clone as 11264-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11264-1-AP (ATG12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)

    IF Staining of PC-3 using 11264-1-AP (same clone as 11264-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 11264-1-AP (ATG12 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

  • WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

    WB analysis using 11264-1-AP (same clone as 11264-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

  • WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)

    WB analysis of HCT 116 using 11264-1-AP (same clone as 11264-1-PBS)

    HCT 116 cells were subjected to SDS PAGE followed by western blot with 11264-1-AP (ATG12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11264-1-PBS in a different storage buffer formulation.

产品信息

11264-1-PBS targets ATG12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATG12 fusion protein Ag1791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Synonyms
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 48-55 kDa
GenBank Accession NumberBC012266
Gene Symbol ATG12
Gene ID (NCBI) 9140
RRIDAB_10807096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94817
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.