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ATG12 Polyclonal antibody

ATG12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30505-1-AP

Print datasheet

Synonyms

FBR93, Autophagy-related protein 12, APG12-like, APG12L, APG12 like

验证数据展示

  • WB analysis using 30505-1-AP

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30505-1-AP

    WB analysis using 30505-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30505-1-AP (ATG12 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, NIH/3T3 cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30505-1-AP targets ATG12 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ATG12 fusion protein Ag32268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATG12 autophagy related 12 homolog (S. cerevisiae)
Synonyms FBR93, Autophagy-related protein 12, APG12-like, APG12L, APG12 like
Calculated Molecular Weight 15 kDa
Observed Molecular Weight20 kDa, 50-60 kDa
GenBank Accession NumberBC012266
Gene Symbol ATG12
Gene ID (NCBI) 9140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94817
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATG12, also named as APG12 and APG12L, belongs to the ATG12 family. It is required for autophagy. Free ATG12 is 15kd or 8kd. Conjugated to ATG5, ATG12-ATG5 show 48-55kd.

实验方案

Product Specific Protocols
WB protocol for ATG12 antibody 30505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols